Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis
Электронный архив ТПУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis
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| Автор |
Khristunova, Ekaterina
Korotkova, Elena Ivanovna Kratochvil, Bohumil Barek, Jiri Dorozhko, Elena Vladimirovna Vyskocil, Vlastimil Plotnikov, Evgeny Vladimirovich Voronova, Olesya Aleksandrovna Sidelnikov, Vladimir Sergeevich |
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| Тематика |
bioconjugates
silver nanoparticles antibodies tick-borne encephalitis virus gold–carbon composite electrode electrochemical immunosensor биоконъюгаты наночастицы антитела вирусы |
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| Описание |
A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies (Ab@AgNP). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their Ab@AgNP. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for Ab@AgNP preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL?1 to 1600 IU·mL?1, with a detection limit of 50 IU·mL?1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids.
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| Дата |
2020-01-17T05:43:24Z
2020-01-17T05:43:24Z 2019 |
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| Тип |
Article
Published version (info:eu-repo/semantics/publishedVersion) Journal article (info:eu-repo/semantics/article) |
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| Идентификатор |
Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis / E. Khristunova [et al.] // Sensors. — 2019. — Vol. 19, iss. 9. — [2103, 10 p.].
http://earchive.tpu.ru/handle/11683/57327 10.3390/s19092103 |
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| Язык |
en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
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| Формат |
application/pdf
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| Издатель |
Томский политехнический университет
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| Источник |
Sensors
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