FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation
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| Автор |
Saraeva, I.
Tolordava, E. Sheligyna, S. Nastulyavichus, A. Khmelnitskii, R. Pokryshkin, N. Khmelenin, D. Kudryashov, S. Ionin, A. Akhmatkhanov, A. |
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| Тематика |
BACTERIA
FOURIER-TRANSFORM IR SPECTROSCOPY IR LASER INACTIVATION CARBON DISTILLED WATER LIPID LIPOPOLYSACCHARIDE MEMBRANE PROTEIN NANOCRYSTAL PEPTIDOGLYCAN SODIUM CHLORIDE ABSORPTION ARTICLE BACTERIAL CELL BACTERIAL MEMBRANE BACTERIAL VIABILITY BACTERIUM CULTURE COLONY FORMING UNIT CONTROLLED STUDY CRYSTALLIZATION FEMTOSECOND INFRARED LASER RADIATION FOURIER TRANSFORM INFRARED SPECTROSCOPY HYDROGEN BOND INFRARED RADIATION LASER THERAPY NONHUMAN OPTICAL DENSITY PROTEIN SECONDARY STRUCTURE PSEUDOMONAS AERUGINOSA ROOM TEMPERATURE SCANNING ELECTRON MICROSCOPY TRANSMISSION ELECTRON MICROSCOPY X RAY DIFFRACTION BACTERIUM CELL MEMBRANE INFRARED SPECTROSCOPY PROCEDURES BACTERIA CELL MEMBRANE MEMBRANE PROTEINS PSEUDOMONAS AERUGINOSA SPECTROSCOPY, FOURIER TRANSFORM INFRARED |
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| Описание |
We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 μm and 6.04 μm, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells in these spectral ranges: vibrations of amide groups in proteins (1500–1700 cm−1), and C-H vibrations in membrane proteins and lipids (2800–3000 cm−1). The underlying bactericidal structural molecular changes were revealed by the stationary Fourier-transform IR spectroscopy, with the spectral peaks parameters being obtained by Lorentzian fitting with the hidden peaks revealed by the second derivative calculations, while no visible damage to the cell membranes was identified by scanning and transmission electron microscopy. © 2023 by the authors.
Ministry of Education and Science of the Russian Federation, Minobrnauka This research was funded by the Ministry of Science and Higher Education of the Russian Federation (Ural Federal University Program of Development within the Priority-2030 Program). |
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| Дата |
2024-04-05T16:18:12Z
2024-04-05T16:18:12Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Saraeva, I, Tolordava, E, Sheligyna, S, Nastulyavichus, A, Khmelnitskii, R, Pokryshkin, N, Khmelenin, D, Kudryashov, S, Ionin, A & Akhmatkhanov, A 2023, 'FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation', International Journal of Molecular Sciences, Том. 24, № 6, 5119. https://doi.org/10.3390/ijms24065119
Saraeva, I., Tolordava, E., Sheligyna, S., Nastulyavichus, A., Khmelnitskii, R., Pokryshkin, N., Khmelenin, D., Kudryashov, S., Ionin, A., & Akhmatkhanov, A. (2023). FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation. International Journal of Molecular Sciences, 24(6), [5119]. https://doi.org/10.3390/ijms24065119 1661-6596 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151110565&doi=10.3390%2fijms24065119&partnerID=40&md5=708532140313d2c8ce0e2c82c2b33d52 https://www.mdpi.com/1422-0067/24/6/5119/pdf?version=1678189578 http://elar.urfu.ru/handle/10995/130305 10.3390/ijms24065119 85151110565 000955341800001 |
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| Язык |
en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
International Journal of Molecular Sciences
International Journal of Molecular Sciences |
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