Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination
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| Автор |
Kulikova, T.
Shiabiev, I. Padnya, P. Rogov, A. Evtugyn, G. Stoikov, I. Porfireva, A. |
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| Тематика |
DOXORUBICIN DETERMINATION
ELECTROCHEMICAL DNA SENSOR ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY ELECTROPOLYMERIZATION PHENOTHIAZINE DERIVATIVE THIACALIX[4]ARENE ACID CHEMICAL DETECTION CHEMOTHERAPY DIAGNOSIS DNA ELECTROCHEMICAL SENSORS ELECTROPOLYMERIZATION GLASS MEMBRANE ELECTRODES MAMMALS MOLAR RATIO DNA SENSORS DOXORUBICIN DOXORUBICIN DETERMINATION ELECTROCHEMICAL DNA SENSOR ELECTROCHEMICAL-IMPEDANCE SPECTROSCOPIES ELECTROPOLYMERISATION PHENOTHIAZINE DERIVATIVES TETRAACIDS THIACALIX[4]ARENE THIACALIX[4]ARENE ACID ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY CARBON CARBOXYLIC ACID DERIVATIVE DOXORUBICIN N PHENYLAMINOPHENOTHIAZINE PHENOTHIAZINE DERIVATIVE THIACALIX[4]ARENE CARBOXYLIC ACID DERIVATIVE UNCLASSIFIED DRUG DNA DOXORUBICIN PHENOTHIAZINE ARTICLE ATOMIC FORCE MICROSCOPY CHEMICAL INTERACTION CONTROLLED STUDY CYCLIC VOLTAMMETRY DNA DETERMINATION DRUG DETERMINATION ELECTROCHEMISTRY ELECTROLYSIS ELECTRON TRANSPORT IMMOBILIZATION IMPEDANCE SPECTROSCOPY LIMIT OF DETECTION MORPHOLOGY POLYMERIZATION VOLTAMMETRY CHEMISTRY ELECTROCHEMICAL ANALYSIS GENETIC PROCEDURES OXIDATION REDUCTION REACTION PROCEDURES BIOSENSING TECHNIQUES CARBON DNA DOXORUBICIN ELECTROCHEMICAL TECHNIQUES ELECTRODES OXIDATION-REDUCTION |
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| Описание |
Electrochemical DNA sensors are highly demanded for fast and reliable determination of antitumor drugs and chemotherapy monitoring. In this work, an impedimetric DNA sensor has been developed on the base of a phenylamino derivative of phenothiazine (PhTz). A glassy carbon electrode was covered with electrodeposited product of PhTz oxidation obtained through multiple scans of the potential. The addition of thiacalix[4]arene derivatives bearing four terminal carboxylic groups in the substituents of the lower rim improved the conditions of electropolymerization and affected the performance of the electrochemical sensor depending on the configuration of the macrocyclic core and molar ratio with PhTz molecules in the reaction medium. Following that, the deposition of DNA by physical adsorption was confirmed by atomic force microscopy and electrochemical impedance spectroscopy. The redox properties of the surface layer obtained changed the electron transfer resistance in the presence of doxorubicin due to its intercalating DNA helix and influencing charge distribution on the electrode interface. This made it possible to determine 3 pM–1 nM doxorubicin in 20 min incubation (limit of detection 1.0 pM). The DNA sensor developed was tested on a bovine serum protein solution, Ringer–Locke’s solution mimicking plasma electrolytes and commercial medication (doxorubicin-LANS) and showed a satisfactory recovery rate of 90–105%. The sensor could find applications in pharmacy and medical diagnostics for the assessment of drugs able to specifically bind to DNA. © 2023 by the authors.
Russian Science Foundation, RSF; Council on grants of the President of the Russian Federation: 19-73-10134 This work was financially supported by Russian Science Foundation, Russian Federation (grant № 19-73-10134, https://www.rscf.ru/en/project/19-73-10134/ (accessed on 2 April 2023)). |
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| Дата |
2024-04-05T16:22:54Z
2024-04-05T16:22:54Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Kulikova, T, Shiabiev, I, Padnya, P, Rogov, A, Evtugyn, G, Stoikov, I & Porfireva, A 2023, 'Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination', Biosensors, Том. 13, № 5, 513. https://doi.org/10.3390/bios13050513
Kulikova, T., Shiabiev, I., Padnya, P., Rogov, A., Evtugyn, G., Stoikov, I., & Porfireva, A. (2023). Impedimetric DNA Sensor Based on Electropolymerized N-Phenylaminophenothiazine and Thiacalix[4]arene Tetraacids for Doxorubicin Determination. Biosensors, 13(5), [513]. https://doi.org/10.3390/bios13050513 2079-6374 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160328582&doi=10.3390%2fbios13050513&partnerID=40&md5=ef7086e0062fe2b2664532026f1bedd5 https://www.mdpi.com/2079-6374/13/5/513/pdf?version=1683280622 http://elar.urfu.ru/handle/10995/130509 10.3390/bios13050513 85160328582 000995519300001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//19-73-10134
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Biosensors
Biosensors |
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