Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
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Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry
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| Автор |
Obluchinskaya, E. D.
Pozharitskaya, O. N. Shevyrin, V. A. Kovaleva, E. G. Flisyuk, E. V. Shikov, A. N. |
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| Тематика |
ALGA
ARCTIC EXTRACTION FUCUS VESICULOSUS NADES NATURAL DEEP EUTECTIC SOLVENT PHLOROTANNINS RESPONSE SURFACE METHODOLOGY (RSM) SEAWEED ALGAL EXTRACT CHOLINE DEEP EUTECTIC SOLVENT HEPTAMER HEXAMER LACTIC ACID NONAMER OCTAMER PENTAMER PHLOROGLUCINOL PHLOROTANNIN DERIVATIVE TANNIN DERIVATIVE TETRAMER TRIMER UNCLASSIFIED DRUG WATER DEEP EUTECTIC SOLVENT PLANT EXTRACT SOLVENT ANTIOXIDANT ACTIVITY ARTICLE CHEMICAL FINGERPRINTING CONCENTRATION (PARAMETER) CONTROLLED STUDY DPPH RADICAL SCAVENGING ASSAY DRUG ISOLATION DRUG STRUCTURE DRY WEIGHT EXPERIMENTAL DESIGN EXTRACTION TIME FUCUS VESICULOSUS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY NONHUMAN RESPONSE SURFACE METHOD TANDEM MASS SPECTROMETRY ULTRASOUND ASSISTED EXTRACTION CHEMISTRY FUCUS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY PROCEDURES CHROMATOGRAPHY, HIGH PRESSURE LIQUID DEEP EUTECTIC SOLVENTS FUCUS PLANT EXTRACTS SOLVENTS TANDEM MASS SPECTROMETRY |
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| Описание |
Phlorotannins are secondary metabolites produced mainly by brown seaweeds (Phaeophyceae) and belong to the class of polyphenolic compounds with diverse bioactivities. The key factors in the extraction of polyphenols are the selection of a suitable solvent, method of extraction and selection of optimal conditions. Ultrasonic-assisted extraction (UAE) is one of the advanced energy-saving methods suitable for the extraction of labile compounds. Methanol, acetone, ethanol and ethyl acetate are the most commonly used solvents for polyphenol extraction. As alternatives to toxic organic solvents, a new class of green solvents, natural deep eutectic solvents (NADES), has been proposed for the efficient extraction of a wide range of natural compounds including polyphenols. Several NADES were screened previously for the extraction of phlorotannins; however, the extraction conditions were not optimized and chemical profiling of NADES extract was not performed. The purpose of this work was to study the effect of selected extraction parameters on the phlorotannin content in NADES extract from Fucus vesiculosus, optimization of extraction conditions and chemical profiling of phlorotannins in the NADES extract. A fast and green NADES-UAE procedure was developed for the extraction of phlorotannins. Optimization was performed through an experimental design and showed that NADES (lactic acid:choline chloride; 3:1) provides a high yield (137.3 mg phloroglucinol equivalents per g dry weight of algae) of phlorotannins under the following extraction conditions: extraction time 23 min, 30.0% water concentration and 1:12 sample to solvent ratio. The antioxidant activity of the optimized NADES extract was equal to that of EtOH extract. In total, 32 phlorotannins have been identified (one trimer, two tetramers, six pentamers, four hexamers, six heptamers, six octamers and seven nonamers) in NADES extracts from arctic F. vesiculosus using the HPLC-HRMS and MS/MS technique. It was noted that all the above-mentioned phlorotannins were identified in both EtOH and NADES extracts. Our results suggest that NADES could be considered as an alternative to the conventional techniques for the effective extraction of phlorotannins from F. vesiculosus with high antioxidant potential. © 2023 by the authors.
121091600104-7; Ministry of Education and Science of the Russian Federation, Minobrnauka; Russian Science Foundation, RSF: 20-66-47017 This study was funded by the Ministry of Science and Higher Education of the Russian Federation within the framework of the Government Assignment to the Murmansk Marine Biological Institute Russian Academy of Sciences (State Reg. No. 121091600104-7). HPLC-HRMS and MS/MS analysis were performed with the financial support from the Russian Science Foundation (Grant 20-66-47017). |
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| Дата |
2024-04-05T16:23:03Z
2024-04-05T16:23:03Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Obluchinskaya, ED, Pozharitskaya, ON, Shevyrin, VA, Kovaleva, EG, Flisyuk, EV & Shikov, AN 2023, 'Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry', Marine Drugs, Том. 21, № 5, 263. https://doi.org/10.3390/md21050263
Obluchinskaya, E. D., Pozharitskaya, O. N., Shevyrin, V. A., Kovaleva, E. G., Flisyuk, E. V., & Shikov, A. N. (2023). Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry. Marine Drugs, 21(5), [263]. https://doi.org/10.3390/md21050263 1660-3397 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160347349&doi=10.3390%2fmd21050263&partnerID=40&md5=95426a5d5520570bd98a271bc5827c9c https://www.mdpi.com/1660-3397/21/5/263/pdf?version=1682335142 http://elar.urfu.ru/handle/10995/130512 10.3390/md21050263 85160347349 000996927100001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//20-66-47017
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Marine Drugs
Marine Drugs |
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