Electrochemical DNA-Sensor Based on Macrocyclic Dendrimers with Terminal Amino Groups and Carbon Nanomaterials
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Electrochemical DNA-Sensor Based on Macrocyclic Dendrimers with Terminal Amino Groups and Carbon Nanomaterials
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| Автор |
Kulikova, T.
Shamagsumova, R. Rogov, A. Stoikov, I. Padnya, P. Shiabiev, I. Evtugyn, G. |
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| Тематика |
CYCLIC VOLTAMMETRY
DNA SENSING DOXORUBICIN ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY SUPRAMOLECULAR DENDRIMERS THIACALIX[4]ARENE DENDRIMERS DNA ELECTROCHEMICAL BIOSENSORS ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY GLASS MEMBRANE ELECTRODES MULTIWALLED CARBON NANOTUBES (MWCN) SCANNING ELECTRON MICROSCOPY DNA SENSING DNA SENSORS DOXORUBICIN ELECTROCHEMICAL DNA SENSOR ELECTROCHEMICAL-IMPEDANCE SPECTROSCOPIES MACROCYCLICS MULTI-WALLED-CARBON-NANOTUBES REDOX INDICATOR SUPRAMOLECULAR DENDRIMERS THIACALIX[4]ARENE CYCLIC VOLTAMMETRY CARBON NANOTUBE DENDRIMER DNA DOXORUBICIN NANOMATERIAL HUMAN DENDRIMERS DNA DOXORUBICIN HUMANS NANOSTRUCTURES NANOTUBES, CARBON |
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| Описание |
The assembling of thiacalix[4]arene-based dendrimers in cone, partial cone, and 1,3-alternate configuration on the surface of a glassy carbon electrode coated with carbon black or multiwalled carbon nanotubes has been characterized using cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. Native and damaged DNA were electrostatically accumulated on the modifier layer. The influence of the charge of the redox indicator and of the macrocycle/DNA ratio was quantified and the roles of the electrostatic interactions and of the diffusional transfer of the redox indicator to the electrode interface indicator access were established. The developed DNA sensors were tested on discrimination of native, thermally denatured, and chemically damaged DNA and on the determination of doxorubicin as the model intercalator. The limit of detection of doxorubicin established for the biosensor based on multi-walled carbon nanotubes was equal to 1.0 pM with recovery from spiked human serum of 105–120%. After further optimization of the assembling directed towards the stabilization of the signal, the developed DNA sensors can find application in the preliminary screening of antitumor drugs and thermal damage of DNA. They can also be applied for testing potential drug/DNA nanocontainers as future delivery systems. © 2023 by the authors.
Russian Science Foundation, RSF: 21-73-20067 This research was funded by the Russian Science Foundation (grant 21-73-20067, https://rscf.ru/en/project/21-73-20067 (accessed on 3 May 2023)). |
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| Дата |
2024-04-05T16:24:11Z
2024-04-05T16:24:11Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Kulikova, T, Shamagsumova, R, Rogov, A, Stoikov, I, Padnya, P, Shiabiev, I & Evtugyn, G 2023, 'Electrochemical DNA-Sensor Based on Macrocyclic Dendrimers with Terminal Amino Groups and Carbon Nanomaterials', Sensors, Том. 23, № 10, 4761. https://doi.org/10.3390/s23104761
Kulikova, T., Shamagsumova, R., Rogov, A., Stoikov, I., Padnya, P., Shiabiev, I., & Evtugyn, G. (2023). Electrochemical DNA-Sensor Based on Macrocyclic Dendrimers with Terminal Amino Groups and Carbon Nanomaterials. Sensors, 23(10), [4761]. https://doi.org/10.3390/s23104761 1424-8220 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160419158&doi=10.3390%2fs23104761&partnerID=40&md5=80c81623dac096cb7d3e03b118adb0ce https://www.mdpi.com/1424-8220/23/10/4761/pdf?version=1684144098 http://elar.urfu.ru/handle/10995/130522 10.3390/s23104761 85160419158 000997384200001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//21-73-20067
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Sensors
Sensors |
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