The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats
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| Автор |
Butova, X.
Myachina, T. Simonova, R. Kochurova, A. Mukhlynina, E. Kopylova, G. Shchepkin, D. Khokhlova, A. |
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| Тематика |
([CA2+]I) TRANSIENTS
ACTIN-MYOSIN INTERACTION ATRIAL FIBRILLATION AUXOTONIC FORCE LEFT AND RIGHT ATRIA PROTEIN PHOSPHORYLATION SARCOMERE SHORTENING SINGLE CARDIOMYOCYTES ACETYLCHOLINE ACTIN CALCIUM ION CARBON FIBER CARDIAC MYOSIN CONTRACTILE PROTEIN GLYCOGEN MYOSIN BINDING PROTEIN C NITRIC OXIDE REACTIVE OXYGEN METABOLITE TROPONIN I TROPONIN T ACTIN MYOSIN INTERACTION ANIMAL EXPERIMENT ANIMAL MODEL ANIMAL TISSUE ARTICLE ATRIAL FIBRILLATION CELL VIABILITY CONFOCAL LASER SCANNING MICROSCOPY CONTROLLED STUDY DEPRESSION ELECTROCARDIOGRAPHY ELECTROPHYSIOLOGY GENE EXPRESSION HEART ATRIUM CONTRACTILITY HEART DEVELOPMENT HEART MUSCLE CONTRACTILITY HISTOLOGY MORPHOLOGY NONHUMAN OXIDATIVE PHOSPHORYLATION PAROXYSMAL ATRIAL FIBRILLATION PROTEIN PHOSPHORYLATION RAT SARCOMERE SARCOMERE LENGTH SARCOPLASMIC RETICULUM SINUS RHYTHM TACHYCARDIA |
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| Описание |
Introduction: The left and right atria (LA, RA) work under different mechanical and metabolic environments that may cause an intrinsic inter-chamber diversity in structure and functional properties between atrial cardiomyocytes (CM) in norm and provoke their different responsiveness to pathological conditions. In this study, we assessed a LA vs. RA difference in CM contractility in paroxysmal atrial fibrillation (AF) and underlying mechanisms. Methods: We investigated the contractile function of single isolated CM from LA and RA using a 7-day acetylcholine (ACh)-CaCl2 AF model in rats. We compared auxotonic force, sarcomere length dynamics, cytosolic calcium ([Ca2+]i) transients, intracellular ROS and NO production in LA and RA CM, and analyzed the phosphorylation levels of contractile proteins and actin-myosin interaction using an in vitro motility assay. Results: AF resulted in more prominent structural and functional changes in LA myocardium, reducing sarcomere shortening amplitude, and velocity of sarcomere relengthening in mechanically non-loaded LA CM, which was associated with the increased ROS production, decreased NO production, reduced myofibrillar content, and decreased phosphorylation of cardiac myosin binding protein C and troponin I. However, in mechanically loaded CM, AF depressed the auxotonic force amplitude and kinetics in RA CM, while force characteristics were preserved in LA CM. Discussion: Thus, inter-atrial differences are increased in paroxysmal AF and affected by the mechanical load that may contribute to the maintenance and progression of AF. 2023 Butova, Myachina, Simonova, Kochurova, Mukhlynina, Kopylova, Shchepkin and Khokhlova.
Russian Science Foundation, RSF: 22-75-10134 This research was supported by the Russian Science Foundation #22-75-10134. The work was performed using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. This research was supported by the Russian Science Foundation #22-75-10134. The work was performed using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. |
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| Дата |
2024-04-05T16:31:29Z
2024-04-05T16:31:29Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Butova, X, Myachina, T, Simonova, R, Kochurova, A, Mukhlynina, E, Kopylova, G, Shchepkin, D & Khokhlova, A 2023, 'The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats', Frontiers in Cardiovascular Medicine, Том. 10, 1203093. https://doi.org/10.3389/fcvm.2023.1203093
Butova, X., Myachina, T., Simonova, R., Kochurova, A., Mukhlynina, E., Kopylova, G., Shchepkin, D., & Khokhlova, A. (2023). The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats. Frontiers in Cardiovascular Medicine, 10, [1203093]. https://doi.org/10.3389/fcvm.2023.1203093 2297-055X Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85168382468&doi=10.3389%2ffcvm.2023.1203093&partnerID=40&md5=8a0ef2fcc5dabc239a93d50f810c19e6 https://www.frontiersin.org/articles/10.3389/fcvm.2023.1203093/pdf http://elar.urfu.ru/handle/10995/130723 10.3389/fcvm.2023.1203093 85168382468 001051362400001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//22-75-10134
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
Frontiers Media SA
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| Источник |
Frontiers in Cardiovascular Medicine
Frontiers in Cardiovascular Medicine |
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