Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite
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| Автор |
Malanina, A.
Kuzin, Y. Khadieva, A. Shibaeva, K. Padnya, P. Stoikov, I. Evtugyn, G. |
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| Тематика |
DNA INTERCALATION
DNA SENSOR ELECTROPOLYMERIZATION PHENOTHIAZINE ELECTROPOLYMERIZATION SELF-ASSEMBLING |
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| Описание |
A novel voltammetric sensor based on a self-assembled composite formed by native DNA and electropolymerized N-phenyl-3-(phenylimino)-3H-phenothiazin-7-amine has been developed and applied for sensitive determination of doxorubicin, an anthracycline drug applied for cancer therapy. For this purpose, a monomeric phenothiazine derivative has been deposited on the glassy carbon electrode from the 0.4 M H2SO4-acetone mixture (1:1 v/v) by multiple potential cycling. The DNA aliquot was either on the electrode modified with electropolymerized film or added to the reaction medium prior to electropolymerization. The DNA entrapment and its influence on the redox behavior of the underlying layer were studied by scanning electron microscopy and electrochemical impedance spectroscopy. The DNA–doxorubicin interactions affected the charge distribution in the surface layer and, hence, altered the redox equilibrium of the polyphenothiazine coating. The voltametric signal was successfully applied for the determination of doxorubicin in the concentration range from 10 pM to 0.2 mM (limit of detection 5 pM). The DNA sensor was tested on spiked artificial plasma samples and two commercial medications (recovery of 90–95%). After further testing on real clinical samples, the electrochemical DNA sensor developed can find application in monitoring drug release and screening new antitumor drugs able to intercalate DNA. © 2023 by the authors.
Russian Science Foundation, RSF; Council on grants of the President of the Russian Federation: 19-73-10134 This work was financially supported by Russian Science Foundation, Russian Federation (grant № 19-73-10134, https://rscf.ru/en/project/19-73-10134/ (accessed on 13 August 2023)). |
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| Дата |
2024-04-05T16:31:55Z
2024-04-05T16:31:55Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Malanina, A, Kuzin, Y, Khadieva, A, Shibaeva, K, Padnya, P, Stoikov, I & Evtugyn, G 2023, 'Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite', Nanomaterials, Том. 13, № 16, 2369. https://doi.org/10.3390/nano13162369
Malanina, A., Kuzin, Y., Khadieva, A., Shibaeva, K., Padnya, P., Stoikov, I., & Evtugyn, G. (2023). Voltammetric Sensor for Doxorubicin Determination Based on Self-Assembled DNA-Polyphenothiazine Composite. Nanomaterials, 13(16), [2369]. https://doi.org/10.3390/nano13162369 2079-4991 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85168895742&doi=10.3390%2fnano13162369&partnerID=40&md5=dd5f6ef4e90bdd52c6099b31744a2381 https://www.mdpi.com/2079-4991/13/16/2369/pdf?version=1692360438 http://elar.urfu.ru/handle/10995/130743 10.3390/nano13162369 85168895742 001056972900001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//19-73-10134
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
Multidisciplinary Digital Publishing Institute (MDPI)
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| Источник |
Nanomaterials
Nanomaterials |
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