Genetic characterization and phylogenetic analysis of human norovirus infection in individual municipalities of the Sverdlovsk region in 2022
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
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Genetic characterization and phylogenetic analysis of human norovirus infection in individual municipalities of the Sverdlovsk region in 2022
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| Автор |
Bykov, R. O.
Scriabina, S. V. Kilyachina, A. S. Itani, T. M. Chalapa, V. I. Starikova, P. K. Koltunov, S. V. Ponomareva, A. V. Semenov, A. V. |
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| Тематика |
GENOGROUP GII
GENOTYPING NOROVIRUSES PHYLOGENETIC ANALYSIS |
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| Описание |
Introduction. Human Noroviruses (HuNoV) are highly contagious pathogens responsible of acute human norovirus infection (HuNoVI). GII.4 is the prevailing norovirus genotype in the incidence of gastroenteritis worldwide. Currently there are no studies on the molecular monitoring and phylogenetic analysis of HuNoV in the territory of the Sverdlovsk region, therefore it is not possible to objectively assess their genetic diversity. The aim of the study is to carry out genotyping and phylogenetic analysis of HNoVI in municipalities of the Sverdlovsk region: Ekaterinburg, Nizhny Tagil, Kamensk-Uralsky in 2022. Materials and methods. Fecal samples were collected from children suffering from HuNoVI in the municipalities of the Sverdlovsk region. Viral RNA was extracted from stool samples followed by reverse transcription polymerase chain reaction with degenerate primers flanking the ORF1/ORF2 junction. The amplified samples were genotyped by Sanger sequencing. Multiple sequence alignments were carried out and phylogenetic trees were constructed. Results. The total number of studied samples was 220. According to sequencing results, 73 positive sequences of HuNoV were typed, which represents 33% of the total volume of the studied samples. The largest share of genotypes is occupied by noroviruses GII (58%), noroviruses GI (42%). Noroviruses GII.17, GI.3 were the dominant genotypes. Phylogenetic analysis demonstrates that the identified sequences on the territory of the Sverdlovsk region have the smallest genetic distance, which gives grounds for their unification into a common cluster. Conclusion. For the first time, a genetic analysis of HuNoV was carried out in the territory of the Sverdlovsk region. The norovirus genotyping system based on the amplification of the ORF1/ORF2 region makes it possible to successfully identify various genotypes of noroviruses from the Sverdlovsk region. It has been confirmed that GII noroviruses remain the dominant genetic group. The results of phylogenetic analysis demonstrate the greatest homology in the formed clusters between isolates from Ekaterinburg, Nizhny Tagil, Kamensk-Uralsky. © Bykov R.O., Scriabina S.V., Kilyachina A.S., Itani T.M., Chalapa V.I., Starikova P.K., Koltunov S.V., Ponomareva A.V., Semenov A.V., 2023.
NIR 123051100045-0 The study was carried out within the framework of the state task of the FNIIVI "Virom" of Rospotrebnadzor (registration No. NIR 123051100045-0). |
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| Дата |
2024-04-05T16:34:57Z
2024-04-05T16:34:57Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Быков, РО, Скрябина, СВ, Килячина, АС, Итани, ТМ, Чалапа, ВИ, Старикова, ПК, Колтунов, СС, Пономарева, АВ & Семенов, АВ 2023, 'МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА И ФИЛОГЕНЕТИЧЕСКИЙ АНАЛИЗ ВОЗБУДИТЕЛЕЙ НОРОВИРУСНОЙ ИНФЕКЦИИ ЧЕЛОВЕКА ОТДЕЛЬНЫХ МУНИЦИПАЛИТЕТОВ В СВЕРДЛОВСКОЙ ОБЛАСТИ ЗА 2022 ГОД', Журнал микробиологии, эпидемиологии и иммунобиологии, Том. 100, № 4, стр. 306-313. https://doi.org/10.36233/0372-9311-402
Быков, Р. О., Скрябина, С. В., Килячина, А. С., Итани, Т. М., Чалапа, В. И., Старикова, П. К., Колтунов, С. С., Пономарева, А. В., & Семенов, А. В. (2023). МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА И ФИЛОГЕНЕТИЧЕСКИЙ АНАЛИЗ ВОЗБУДИТЕЛЕЙ НОРОВИРУСНОЙ ИНФЕКЦИИ ЧЕЛОВЕКА ОТДЕЛЬНЫХ МУНИЦИПАЛИТЕТОВ В СВЕРДЛОВСКОЙ ОБЛАСТИ ЗА 2022 ГОД. Журнал микробиологии, эпидемиологии и иммунобиологии, 100(4), 306-313. https://doi.org/10.36233/0372-9311-402 0372-9311 Final All Open Access, Gold https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175003885&doi=10.36233%2f0372-9311-402&partnerID=40&md5=d0ff977c0c2dc7e46a5a2bdd73752791 https://microbiol.crie.ru/jour/article/download/9992/1324 http://elar.urfu.ru/handle/10995/130885 54510115 10.36233/0372-9311-402 85175003885 |
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| Язык |
ru
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
Central Research Institute for Epidemiology
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| Источник |
Journal of microbiology, epidemiology and immunobiology
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii |
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