Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent
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| Автор |
Porfireva, A.
Begisheva, E. Evtugyn, V. Evtugyn, G. |
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| Тематика |
AZURE C
DEEP EUTECTIC SOLVENT DNA SENSOR ELECTROCHEMICAL SENSOR ELECTROPOLYMERIZATION VALRUBICIN DETERMINATION BIOSENSORS CHEMICAL DETECTION DNA ELECTROCHEMICAL BIOSENSORS ELECTROCHEMICAL ELECTRODES ELECTROPOLYMERIZATION EUTECTICS REDOX REACTIONS SOLVENTS ANTHRACYCLINE DRUGS AZURE C C FILMS DEEP EUTECTIC SOLVENTS DNA SENSORS ELECTROCHEMICAL DNA SENSOR ELECTROPOLYMERISATION PHOSPHATE BUFFERS VALRUBICIN VALRUBICIN DETERMINATION COATINGS ANTHRACYCLINE DEEP EUTECTIC SOLVENT REACTIVE OXYGEN METABOLITE VALRUBICIN AZURE C DNA PHOSPHATE SOLVENT VALRUBICIN ARTICLE CONTROLLED STUDY CYCLIC VOLTAMMETRY DRUG DETERMINATION ELECTROCHEMICAL ANALYSIS ELECTROLYTE BLOOD LEVEL ELECTRON TRANSPORT HUMAN LIMIT OF DETECTION PH PHARMACOKINETICS POLYMERIZATION SCANNING ELECTRON MICROSCOPY STOICHIOMETRY THYMUS VOLTAMMETRY REPRODUCIBILITY DEEP EUTECTIC SOLVENTS DNA ELECTROCHEMICAL TECHNIQUES ELECTRODES PHOSPHATES REPRODUCIBILITY OF RESULTS SOLVENTS |
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| Описание |
A novel electrochemical DNA sensor was developed for the detection of the anthracycline drug, valrubicin, on the base of poly(Azure C) electropolymerized from the deep eutectic solvent reline and covered with adsorbed DNA from calf thymus. Biosensor assembling was performed by multiple scanning of the potential in one drop (100 µL) of the dye dissolved in reline and placed on the surface of a screen-printed carbon electrode. Stabilization of the coating was achieved by its polarization in the phosphate buffer. The electrochemical characteristics of the electron transfer were determined and compared with a similar coating obtained from phosphate buffer. The use of deep eutectic solvent made it possible to increase the monomer concentration and avoid using organic solvents on the stage of electrode modification. After the contact of the DNA sensor with valrubicin, two signals related to the intrinsic redox activity of the coating and the drug redox conversion were found on voltammogram. Their synchronous changes with the analyte concentration increased the reliability of the detection. In the square-wave mode, the DNA sensor made it possible to determine from 3 µM to 1 mM (limit of detection, 1 µM) in optimal conditions. The DNA sensor was successfully tested in the voltammetric determination of valrubicin in spiked artificial urine, Ringer-Locke solution mimicking plasma electrolytes and biological samples (urine and saliva) with a recovery of 90–110%. After further testing on clinical samples, it can find application in the pharmacokinetics studies and screening of new drugs’ interaction with DNA. © 2023 by the authors.
Russian Science Foundation, RSF: 23-13-00163 This research was funded by the Russian Science Foundation (grant No. 23-13-00163), https://www.rscf.ru/en/project/23-13-00163 (assessed on 17 August 2023). |
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| Дата |
2024-04-05T16:35:07Z
2024-04-05T16:35:07Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Porfireva, A, Begisheva, E, Evtugyn, V & Evtugyn, G 2023, 'Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent', Biosensors, Том. 13, № 10, 931. https://doi.org/10.3390/bios13100931
Porfireva, A., Begisheva, E., Evtugyn, V., & Evtugyn, G. (2023). Electrochemical DNA Sensor for Valrubicin Detection Based on Poly(Azure C) Films Deposited from Deep Eutectic Solvent. Biosensors, 13(10), [931]. https://doi.org/10.3390/bios13100931 2079-6374 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175077994&doi=10.3390%2fbios13100931&partnerID=40&md5=a2525f0bd47d6a2d8c4caeb0ede22faa https://www.mdpi.com/2079-6374/13/10/931/pdf?version=1697608429 http://elar.urfu.ru/handle/10995/130894 10.3390/bios13100931 85175077994 001094181200001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//23-13-00163
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
Multidisciplinary Digital Publishing Institute (MDPI)
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| Источник |
Biosensors
Biosensors |
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