Biochemical Responses to the Long-Term Impact of Copper Sulfate (CuSO4) in Tobacco Plants
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Biochemical Responses to the Long-Term Impact of Copper Sulfate (CuSO4) in Tobacco Plants
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| Автор |
Tugbaeva, A. S.
Ermoshin, A. A. Kiseleva, I. S. |
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| Тематика |
ANTIOXIDANT ENZYMES ACTIVITY
COPPER STRESS FREE AND BOUND PHENOLICS LIGNIFICATION NICOTIANA TABACUML SECONDARY METABOLITES CINNAMIC ACID COPPER SULFATE FERULIC ACID GUAIACOL PEROXIDASE HYDROGEN PEROXIDE LIGNIN MALONALDEHYDE PARA COUMARIC ACID SUPEROXIDE DISMUTASE ANTIOXIDANT COPPER COPPER SULFATE ARTICLE BIOACCUMULATION CONTROLLED STUDY CYSTEINE ASSISTED SULFURIC METHOD ENZYME ACTIVITY LEAF GROWTH LIGNIFICATION LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY NICOTIANA TABACUM NONHUMAN ORGAN SIZE OXIDATIVE STRESS PHYSIOLOGICAL PROCESS PLANT LEAF PLANT RESPONSE PLANT ROOT PLANT STEM PLANT STRESS SHOOT SPECTROSCOPY THIN LAYER CHROMATOGRAPHY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY METABOLISM TOBACCO ANTIOXIDANTS COPPER COPPER SULFATE HYDROGEN PEROXIDE LIGNIN OXIDATIVE STRESS PLANT ROOTS TOBACCO |
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| Описание |
Metabolic changes under stress are often studied in short-term experiments, revealing rapid responses in gene expression, enzyme activity, and the amount of antioxidants. In a long-term experiment, it is possible to identify adaptive changes in both primary and secondary metabolism. In this study, we characterized the physiological state of tobacco plants and assessed the amount and spectrum of phenolic compounds and the lignification of axial organs under excess copper stress in a long-term experiment (40 days). Plants were treated with 100 and 300 μM CuSO4, as well as a control (Knop solution). Copper accumulation, the size and anatomical structure of organs, stress markers, and the activity of antioxidant enzymes were studied. Lignin content was determined with the cysteine-assisted sulfuric method (CASA), and the metabolite profile and phenolic spectrum were determined with UHPLC-MS and thin-layer chromatography (TLC). Cu2+ mainly accumulated in the roots and, to a lesser extent, in the shoots. Copper sulfate (100 μM) slightly stimulated stem and leaf growth. A higher concentration (300 μM) caused oxidative stress; H2O2 content, superoxide dismutase (SOD), and guaiacol peroxidase (GPOX) activity increased in roots, and malondialdehyde (MDA) increased in all organs. The deposition of lignin increased in the roots and stems compared with the control. The content of free phenolics, which could be used as substrates for lignification, declined. The proportions of ferulic, cinnamic, and p-coumaric acids in the hydrolysate of bound phenolics were higher, and they tended toward additional lignification. The metabolic profile changed in both roots and stems at both concentrations, and changed in leaves only at a concentration of 300 μM. Thus, changes in the phenolic spectrum and the enhanced lignification of cell walls in the metaxylem of axial (root and stem) organs in tobacco can be considered important metabolic responses to stress caused by excess CuSO4. © 2023 by the authors.
Russian Science Foundation, RSF: 22-24-00817 This research was supported by the Russian Science Foundation, project no. 22-24-00817, https://rscf.ru/project/22-24-00817/ accessed on 4 September 2023. |
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| Дата |
2024-04-05T16:35:12Z
2024-04-05T16:35:12Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Tugbaeva, A, Ermoshin, A & Kiseleva, I 2023, 'Biochemical Responses to the Long-Term Impact of Copper Sulfate (CuSO4) in Tobacco Plants', International Journal of Molecular Sciences, Том. 24, № 20, 15129. https://doi.org/10.3390/ijms242015129
Tugbaeva, A., Ermoshin, A., & Kiseleva, I. (2023). Biochemical Responses to the Long-Term Impact of Copper Sulfate (CuSO4) in Tobacco Plants. International Journal of Molecular Sciences, 24(20), [15129]. https://doi.org/10.3390/ijms242015129 1661-6596 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175274156&doi=10.3390%2fijms242015129&partnerID=40&md5=82415e32989d4ba5f94bca6a86a97fee https://www.mdpi.com/1422-0067/24/20/15129/pdf?version=1697167265 http://elar.urfu.ru/handle/10995/130900 10.3390/ijms242015129 85175274156 001097927600001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//22-24-00817
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
Multidisciplinary Digital Publishing Institute (MDPI)
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| Источник |
International Journal of Molecular Sciences
International Journal of Molecular Sciences |
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