Microbial tapestry of the Shulgan-Tash cave (Southern Ural, Russia): influences of environmental factors on the taxonomic composition of the cave biofilms
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Microbial tapestry of the Shulgan-Tash cave (Southern Ural, Russia): influences of environmental factors on the taxonomic composition of the cave biofilms
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| Автор |
Gogoleva, N.
Chervyatsova, O. Balkin, A. Kuzmina, L. Shagimardanova, E. Kiseleva, D. Gogolev, Y. |
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| Тематика |
16S RRNA GENE
BIOFILM CROSSIELLA GA0077536 KARST CAVE NITROSOCOCCACEAE WB1-P19 PALEOLITHIC PAINTING SHULGAN-TASH CALCIUM CARBONATE CARBON DIOXIDE ACTINOBACTERIA AIR CONDITIONING AIR TEMPERATURE AMINO ACID METABOLISM ARTICLE BACTERIAL COLONIZATION BIOFILM CAVE CONTROLLED STUDY DISSOLUTION ENVIRONMENTAL FACTOR ENVIRONMENTAL IMPACT GAMMAPROTEOBACTERIA HUMIDITY KEGG MEASUREMENT ACCURACY MICROBIAL COMMUNITY MINERALIZATION MOISTURE MORPHOLOGY MORPHOTYPE NONHUMAN PAINTING PALEOLITHIC PHYLUM PLANCTOMYCETES PRECIPITATION PREDICTION RELATIVE HUMIDITY RUSSIAN FEDERATION SCANNING ELECTRON MICROSCOPY SPECIES COMPOSITION SPECIES DIVERSITY SUMMER SURFACE PROPERTY TAXONOMY WATER VAPOR |
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| Описание |
Background: Cave biotopes are characterized by stable low temperatures, high humidity, and scarcity of organic substrates. Despite the harsh oligotrophic conditions, they are often inhabited by rich microbial communities. Abundant fouling with a wide range of morphology and coloration of colonies covers the walls of the Shulgan-Tash cave in the Southern Urals. This cave is also famous for the unique Paleolithic painting discovered in the middle of the last century. We aimed to investigate the diversity, distribution, and potential impact of these biofilms on the cave’s Paleolithic paintings, while exploring how environmental factors influence the microbial communities within the cave. Results: The cave’s biofilm morphotypes were categorized into three types based on the ultrastructural similarities. Molecular taxonomic analysis identified two main clusters of microbial communities, with Actinobacteria dominating in most of them and a unique “CaveCurd” community with Gammaproteobacteria prevalent in the deepest cave sections. The species composition of these biofilms reflects changes in environmental conditions, such as substrate composition, temperature, humidity, ventilation, and CO2 content. Additionally, it was observed that cave biofilms contribute to biocorrosion on cave wall surfaces. Conclusions: The Shulgan-Tash cave presents an intriguing example of a stable extreme ecosystem with diverse microbiota. However, the intense dissolution and deposition of carbonates caused by Actinobacteria pose a potential threat to the preservation of the cave’s ancient rock paintings. © 2023, The Author(s).
075- 03-2021-607; Ministry of Education and Science of the Russian Federation, Minobrnauka: 122031100163-4, 122092200006-4, 2-22-91-2; Kazan Federal University: PRIORITY-2030 The research has been supported by the Kazan Federal University Strategic Academic Leadership Program (PRIORITY-2030). The paper publishing has been supported by Leopold-Franzens-Universität Innsbruck, Austria. We thank State Nature Reserve “Shulgan-Tash” represented by Director Dr. Michael Kosarev and Center for Protection and Use of Objects of Cultural Heritage” represented by Director Dannir Gaynullin for the long-term support of research in the Shulgan-Tash cave. We thank Dr. Irek Musabirov and Dr. Semen Sergeev (Institut for Metals Superplasticity Problems RAN, Ufa, Russia) for their assistance in SEM research. We thank Dr. Yuri Dublyansky (Institut für Geologie, Leopold-Franzens-Universität Innsbruck, Austria) for the analysis of C-CO isotopic composition and discussion of microclimatic results. We thank Rayan Akhmedyanov, Nikolay Grigoriev and Avzal Sagitov (“The Cave Shulgan-Tash” historical cultural reserve museum) for help in microclimatic research. We thank Dr. Alexey Potekhin for the inner reviewing of the manuscript (Research Department for Limnology, Leopold-Franzens-Universität Innsbruck, Austria). LK’s work was performed within the framework of the state assignment UIB of the UFIC RAS № 075- 03-2021-607 of the Ministry of Science and Higher Education of the Russian Federation under the topic №122031100163-4. The ICP-MS microelement analysis was conducted in the “Geoanalitik“ shared research facilities of the IGG UB RAS as part of the state assignment (registration number AAAA-A18-118053090045-8).OCh’s work was carried out within the framework of the state assignment of the Ministry of Ecology and Natural Resources of Russia, project number 2-22-91-2, under theme number 122092200006-4. 2 We thank State Nature Reserve “Shulgan-Tash” represented by Director Dr. Michael Kosarev and Center for Protection and Use of Objects of Cultural Heritage” represented by Director Dannir Gaynullin for the long-term support of research in the Shulgan-Tash cave. We thank Dr. Irek Musabirov and Dr. Semen Sergeev (Institut for Metals Superplasticity Problems RAN, Ufa, Russia) for their assistance in SEM research. We thank Dr. Yuri Dublyansky (Institut für Geologie, Leopold-Franzens-Universität Innsbruck, Austria) for the analysis of C-CO2 isotopic composition and discussion of microclimatic results. We thank Rayan Akhmedyanov, Nikolay Grigoriev and Avzal Sagitov (“The Cave Shulgan-Tash” historical cultural reserve museum) for help in microclimatic research. We thank Dr. Alexey Potekhin for the inner reviewing of the manuscript (Research Department for Limnology, Leopold-Franzens-Universität Innsbruck, Austria). LK’s work was performed within the framework of the state assignment UIB of the UFIC RAS № 075- 03-2021-607 of the Ministry of Science and Higher Education of the Russian Federation under the topic №122031100163-4. The ICP-MS microelement analysis was conducted in the “Geoanalitik“ shared research facilities of the IGG UB RAS as part of the state assignment (registration number AAAA-A18-118053090045-8).OCh’s work was carried out within the framework of the state assignment of the Ministry of Ecology and Natural Resources of Russia, project number 2-22-91-2, under theme number 122092200006-4. |
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| Дата |
2024-04-05T16:36:45Z
2024-04-05T16:36:45Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Gogoleva, N, Chervyatsova, O, Balkin, A, Kuzmina, L, Shagimardanova, E, Kiseleva, D & Gogolev, Y 2023, 'Microbial tapestry of the Shulgan-Tash cave (Southern Ural, Russia): influences of environmental factors on the taxonomic composition of the cave biofilms', Environmental Microbiome, Том. 18, № 1, 82. https://doi.org/10.1186/s40793-023-00538-1
Gogoleva, N., Chervyatsova, O., Balkin, A., Kuzmina, L., Shagimardanova, E., Kiseleva, D., & Gogolev, Y. (2023). Microbial tapestry of the Shulgan-Tash cave (Southern Ural, Russia): influences of environmental factors on the taxonomic composition of the cave biofilms. Environmental Microbiome, 18(1), [82]. https://doi.org/10.1186/s40793-023-00538-1 2524-6372 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85177473210&doi=10.1186%2fs40793-023-00538-1&partnerID=40&md5=f5c9d9c4f89121bbe78cfbd2d067edd9 https://environmentalmicrobiome.biomedcentral.com/counter/pdf/10.1186/s40793-023-00538-1 http://elar.urfu.ru/handle/10995/130987 10.1186/s40793-023-00538-1 85177473210 001108210500001 |
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| Язык |
en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
BioMed Central Ltd
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| Источник |
Environmental Microbiome
Environmental Microbiome |
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