Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study
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| Автор |
Dutysheva, E. A.
Mikhaylova, E. R. Trestsova, M. A. Andreev, A. I. Apushkin, D. Y. Utepova, I. A. Serebrennikova, P. O. Akhremenko, E. A. Aksenov, N. D. Bon’, E. I. Zimatkin, S. M. Chupakhin, O. N. Margulis, B. A. Guzhova, I. V. Lazarev, V. F. |
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| Тематика |
3-(5-PHENYL-1H-PYRROL-2-YL)QUINOXALIN-2(1H)-ONE
GAPDH HEAT SHOCK PROTEIN HSP70 HYDROCORTISONE 21-HEMISUCCINATE REHABILITATION THERAPY SECONDARY DAMAGE SMALL MOLECULES TRAUMATIC BRAIN INJURY CHAPERONE GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE HEAT SHOCK PROTEIN 70 HUNTINGTIN HYDROCORTISONE HYDROCORTISONE SODIUM SUCCINATE PENICILLIN DERIVATIVE PYRROLYLAZINE DERIVATIVE STREPTOMYCIN SUPEROXIDE DISMUTASE TILETAMINE PLUS ZOLAZEPAM UNCLASSIFIED DRUG ZOLETIL 100 ALZHEIMER DISEASE ANIMAL CELL ANIMAL EXPERIMENT ANIMAL MODEL APOPTOSIS ARTICLE BRAIN CORTEX CELL AGGREGATION CELL INVASION ASSAY CELL PROLIFERATION CELL PROLIFERATION ASSAY CONFOCAL MICROSCOPY CONTROLLED STUDY FLOW CYTOMETRY GLIOBLASTOMA GLIOMA IMMUNOFLUORESCENCE IMMUNOFLUORESCENCE ASSAY IMMUNOHISTOCHEMISTRY IMPEDANCE INFLAMMATION MORRIS WATER MAZE TEST NERVE DEGENERATION NONHUMAN PILOT STUDY RAT TRAUMATIC BRAIN INJURY TUNEL ASSAY UPREGULATION WESTERN BLOTTING |
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| Описание |
The recovery period after traumatic brain injury (TBI) is often complicated by secondary damage that may last for days or even months after trauma. Two proteins, Hsp70 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were recently described as modulating post-traumatic processes, and in this study, we test them as targets for combination therapy using an inhibitor of GAPDH aggregation (derivative of hydrocortisone RX624) and an inducer of Hsp70 synthesis (the pyrrolylazine derivative PQ-29). The protective effect of the combination on C6 rat glioblastoma cells treated with the cerebrospinal fluid of traumatized animals resulted in an increase in the cell index and in a reduced level of apoptosis. Using a rat weight drop model of TBI, we found that the combined use of both drugs prevented memory impairment and motor deficits, as well as a reduction of neurons and accumulation of GAPDH aggregates in brain tissue. In conclusion, we developed and tested a new approach to the treatment of TBI based on influencing distinct molecular mechanisms in brain cells. © 2022 by the authors.
Ministry of Education and Science of the Russian Federation, Minobrnauka: 075-15-2021-683; Russian Science Foundation, RSF: 18-74-10087, 22-13-00298 This research was funded by the Russian Science Foundation, research projects #18-74-10087 and #22-13-00298. Rat glioblastoma C6 cells were obtained from the shared research facility “Vertebrate cell culture collection” supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2021-683). Cells were cultured in DMEM/F12 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 100 units/mL penicillin, and 0.1 mg/mL streptomycin (BioloT, St. Petersburg, Russia) at 37 °C and 5% CO. 2 |
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| Дата |
2024-04-05T16:37:09Z
2024-04-05T16:37:09Z 2023 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) |info:eu-repo/semantics/publishedVersion |
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| Идентификатор |
Dutysheva, EA, Mikhaylova, ER, Trestsova, MA, Andreev, AI, Apushkin, DY, Utepova, IA, Serebrennikova, PO, Akhremenko, EA, Aksenov, ND, Bon’, EI, Zimatkin, SM, Chupakhin, ON, Margulis, BA, Guzhova, IV & Lazarev, VF 2023, 'Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study', Pharmaceutics, Том. 15, № 1, 7. https://doi.org/10.3390/pharmaceutics15010007
Dutysheva, E. A., Mikhaylova, E. R., Trestsova, M. A., Andreev, A. I., Apushkin, D. Y., Utepova, I. A., Serebrennikova, P. O., Akhremenko, E. A., Aksenov, N. D., Bon’, E. I., Zimatkin, S. M., Chupakhin, O. N., Margulis, B. A., Guzhova, I. V., & Lazarev, V. F. (2023). Combination of a Chaperone Synthesis Inducer and an Inhibitor of GAPDH Aggregation for Rehabilitation after Traumatic Brain Injury: A Pilot Study. Pharmaceutics, 15(1), [7]. https://doi.org/10.3390/pharmaceutics15010007 1999-4923 Final All Open Access, Gold, Green https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146778461&doi=10.3390%2fpharmaceutics15010007&partnerID=40&md5=9dbb477c2158ebf193dadffce65cf6bc https://www.mdpi.com/1999-4923/15/1/7/pdf?version=1672128532 http://elar.urfu.ru/handle/10995/131014 10.3390/pharmaceutics15010007 85146778461 000916065300001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//18-74-10087
info:eu-repo/grantAgreement/RSF//22-13-00298 |
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Pharmaceutics
Pharmaceutics |
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