Biofertilizer Based on Biochar and Metal-Tolerant Plant Growth Promoting Rhizobacteria Alleviates Copper Impact on Morphophysiological Traits in Brassica napus L.
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Biofertilizer Based on Biochar and Metal-Tolerant Plant Growth Promoting Rhizobacteria Alleviates Copper Impact on Morphophysiological Traits in Brassica napus L.
|
|
| Автор |
Kumar, A.
Borisova, G. Maleva, M. Tripti Shiryaev, G. Tugbaeva, A. Sobenin, A. Kiseleva, I. |
|
| Тематика |
BACILLUS ALTITUDINIS
BIOFORMULATION HEAVY METAL LIPID PEROXIDATION MORPHOLOGICAL PARAMETERS NON-ENZYMATIC ANTIOXIDANTS PHOTOSYNTHETIC PIGMENTS RAPESEED |
|
| Описание |
Metal tolerant plant growth-promoting (PGP) rhizobacteria are promising for enhancing plant productivity under copper (Cu) stress. Present pot scale experiment was conducted on Brassica napus L. to check the efficiency of rhizobacteria isolated from the rhizosphere of Tussilago farfara L. growing on Cu-contaminated soils. Out of fifty Cu tolerant strains, three isolates which showed multiple PGP traits such as indole-3-acetic acid (IAA) synthesis, phosphate (PS) solubilization, siderophore and ammonia production were identified preliminarily by morphological and physiological characteristics followed by 16S rRNA gene sequencing. The best Bacillus altitudinis strain TF16a which showed IAA: 15.5 mg L−1, PS: 215 mg L−1, siderophore halo zone ratio of 3.0 with high ammonia production was selected to prepare a biochar-based biofertilizer (BF). Seedling test showed maximum growth of B. napus shoot and root in presence of 5% of BF and this concentration was selected for further experiment. The pot experiment included four treatments: control (soil), 100Cu (100 mg Cu kg−1 soil), 5%BF (v/v), and 5%BF+100Cu, which were carried out for 30 days, after which the morphological, physiological, and biochemical parameters of B. napus were studied. The Cu treatment caused its accumulation in shoot and root up to 16.9 and 30.4 mg kg−1 DW, respectively, and increased malondialdehyde (MDA) content by 20%. Application of BF with copper led to the decrease in the Cu accumulation by 20% for shoot and 28% for root while MDA content was the same as in the control. Both treatments of BF with and without Cu increased chlorophyll a and b content by 1.3 times on average as well as non-enzymatic antioxidants such as soluble phenolic compounds (1.3 times) and free proline (1.6 times). Moreover, BF + Cu led to the increase in the biomass of shoot and root by 30 and 60%, respectively, while there was no significant effect on the growth characteristics of plants after the addition of BF without Cu. The study elucidates that BF based on B. altitudinis strain TF16a and biochar can be a promising bioformulation which could increase rapeseed growth under the moderate Cu concentration in soil. © 2022 by the authors.
Ministry of Education and Science of the Russian Federation, Minobrnauka, (FEUZ-2021-0014) Russian Science Foundation, RSF, (21-76-00011) Ural Federal University, UrFU Funding text 1: The work was supported and funded by Russian Science Foundation (Project No. 21-76-00011), https://rscf.ru/project/21-76-00011 for soil sampling and preparation isolation, morphological characterization and genetic identification of rhizospheric bacteria carrier characterization and biofertilizer preparation and partly (assessment of plant morphological and physiological traits) by the Ministry of Science and Higher Education of the Russian Federation within the framework of State Assignment FEUZ-2021-0014. Funding text 2: The authors acknowledge the work support from UrFU, Yekaterinburg. The authors also acknowledge the multiple anonymous reviewers for their constructive comments. |
|
| Дата |
2024-04-08T11:06:26Z
2024-04-08T11:06:26Z 2022 |
|
| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
|
| Идентификатор |
Kumar, A, Borisova, G, Maleva, M, Tripti, , Shiryaev, G, Tugbaeva, A, Sobenin, A & Kiseleva, I 2022, 'Biofertilizer Based on Biochar and Metal-Tolerant Plant Growth Promoting Rhizobacteria Alleviates Copper Impact on Morphophysiological Traits in Brassica napus L.', Microorganisms, Том. 10, № 11, 2164. https://doi.org/10.3390/microorganisms10112164
Kumar, A., Borisova, G., Maleva, M., Tripti, Shiryaev, G., Tugbaeva, A., Sobenin, A., & Kiseleva, I. (2022). Biofertilizer Based on Biochar and Metal-Tolerant Plant Growth Promoting Rhizobacteria Alleviates Copper Impact on Morphophysiological Traits in Brassica napus L. Microorganisms, 10(11), [2164]. https://doi.org/10.3390/microorganisms10112164 2076-2607 Final All Open Access; Gold Open Access; Green Open Access https://www.mdpi.com/2076-2607/10/11/2164/pdf?version=1667219714 https://www.mdpi.com/2076-2607/10/11/2164/pdf?version=1667219714 http://elar.urfu.ru/handle/10995/131311 10.3390/microorganisms10112164 85141704987 000882264400001 |
|
| Язык |
en
|
|
| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//21-76-00011
|
|
| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
|
| Формат |
application/pdf
|
|
| Издатель |
MDPI
|
|
| Источник |
Microorganisms
Microorganisms |
|