New TEMPO–Appended 2,2′-Bipyridine-Based Eu(III), Tb(III), Gd(III) and Sm(III) Complexes: Synthesis, Photophysical Studies and Testing Photoluminescence-Based Bioimaging Abilities
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Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
New TEMPO–Appended 2,2′-Bipyridine-Based Eu(III), Tb(III), Gd(III) and Sm(III) Complexes: Synthesis, Photophysical Studies and Testing Photoluminescence-Based Bioimaging Abilities
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| Автор |
Slovesnova, N. V.
Minin, A. S. Belousova, A. V. Ustyugov, A. A. Chaprov, K. D. Krinochkin, A. P. Valieva, M. I. Shtaitz, Y. K. Starnovskaya, E. S. Nikonov, I. L. Tsmokalyuk, A. N. Kim, G. A. Santra, S. Kopchuk, D. S. Nosova, E. V. Zyryanov, G. V. |
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| Тематика |
2,2′-BIPYRIDINES
BIOGENIC THIOLS BSA EPR METHODS EU(III) COMPLEX FLUORESCENCE QUENCHING PRE-LUMINESCENCE PROBE TEMPO 2,2'-DIPYRIDYL ALZHEIMER DISEASE ANIMALS CYSTEINE FLUORESCENCE FLUORESCENT DYES GLUTATHIONE LIGANDS MICE SERUM ALBUMIN, BOVINE SULFHYDRYL COMPOUNDS 2,2' BIPYRIDINE BOVINE SERUM ALBUMIN CYSTEINE FLUORESCENT DYE GLUTATHIONE LIGAND THIOL DERIVATIVE ALZHEIMER DISEASE ANIMAL CHEMISTRY FLUORESCENCE MOUSE |
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| Описание |
Linked to Alzheimer’s disease (AD), amyloids and tau-protein are known to contain a large number of cysteine (Cys) residues. In addition, certain levels of some common biogenic thiols (cysteine (Cys), homocysteine (Hcy), glutathione (GSH), etc.) in biological fluids are closely related to AD as well as other diseases. Therefore, probes with a selective interaction with the above-mentioned thiols can be used for the monitoring and visualizing changes of (bio)thiols in the biological fluids as well as in the brain of animal models of Alzheimer’s disease. In this study, new Eu(III), Tb(III), Gd(III) and Sm(III) complexes of 2,2′-bipyridine ligands containing TEMPO fragments as receptor units for (bio)thiols are reported. The presence of free radical fragments of the ligand in the complexes was proved by using the electronic paramagnetic resonance (EPR) method. Among all the complexes, the Eu(III) complex turned out to be the most promising one as luminescence- and spin-probe for the detection of biogenic thiols. The EPR and fluorescent titration methods showed the interaction of the resulting complex with free Cys and GSH in solution. To study the practical applicability of the probes for the monitoring of AD in-vivo, by using the above-mentioned Eu(III)-based probe, the staining of the brain of mice with amyloidosis and Vero cell cultures supplemented with the cysteine-enriched medium was studied as well as the fluorescence titration of Bovine Serum Albumin, BSA (as the model for the thiol moieties containing protein), was carried out. Based on the results of fluorescence titration, the formation of a non-covalent inclusion complex between the above-mentioned Eu(III) complex and BSA was suggested. © 2022 by the authors.
Ministry of Education and Science of the Russian Federation, Minobrnauka, (075-15-2020-777) This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Grant no.: 075-15-2020-777). |
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| Дата |
2024-04-08T11:06:39Z
2024-04-08T11:06:39Z 2022 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
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| Идентификатор |
Slovesnova, NV, Minin, AS, Belousova, AV, Ustyugov, AA, Chaprov, KD, Krinochkin, AP, Valieva, MI, Shtaitz, YK, Starnovskaya, ES, Nikonov, IL, Tsmokalyuk, AN, Kim, GA, Santra, S, Kopchuk, DS, Nosova, EV & Zyryanov, GV 2022, 'New TEMPO–Appended 2,2′-Bipyridine-Based Eu(III), Tb(III), Gd(III) and Sm(III) Complexes: Synthesis, Photophysical Studies and Testing Photoluminescence-Based Bioimaging Abilities', Molecules, Том. 27, № 23, 8414. https://doi.org/10.3390/molecules27238414
Slovesnova, N. V., Minin, A. S., Belousova, A. V., Ustyugov, A. A., Chaprov, K. D., Krinochkin, A. P., Valieva, M. I., Shtaitz, Y. K., Starnovskaya, E. S., Nikonov, I. L., Tsmokalyuk, A. N., Kim, G. A., Santra, S., Kopchuk, D. S., Nosova, E. V., & Zyryanov, G. V. (2022). New TEMPO–Appended 2,2′-Bipyridine-Based Eu(III), Tb(III), Gd(III) and Sm(III) Complexes: Synthesis, Photophysical Studies and Testing Photoluminescence-Based Bioimaging Abilities. Molecules, 27(23), [8414]. https://doi.org/10.3390/molecules27238414 1420-3049 Final All Open Access; Gold Open Access; Green Open Access https://www.mdpi.com/1420-3049/27/23/8414/pdf?version=1670029250 https://www.mdpi.com/1420-3049/27/23/8414/pdf?version=1670029250 http://elar.urfu.ru/handle/10995/131331 10.3390/molecules27238414 85143701524 000898014700001 |
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| Язык |
en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Molecules
Molecules |
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