Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor
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| Автор |
Ivanov, A.
Stoikov, D. Shafigullina, I. Shurpik, D. Stoikov, I. Evtugyn, G. |
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| Тематика |
ACETYLCHOLINESTERASE SENSOR
ELECTROPOLYMERIZATION FLOW-THROUGH ANALYSIS INHIBITOR DETERMINATION PILLAR[5]ARENE ACETYLCHOLINESTERASE BERBERINE BIOSENSING TECHNIQUES CARBOFURAN CARBON DONEPEZIL ELECTRODES ENZYMES, IMMOBILIZED METHYLENE BLUE PHOSPHATES SOOT THIOCHOLINE 3D PRINTING AROMATIC COMPOUNDS BIOACTIVITY CARBON BLACK ELECTRODES ELECTROPOLYMERIZATION ENZYMATIC HYDROLYSIS ENZYME ELECTRODES ACETYLCHOLINESTERASE BERBERINE CARBOFURAN DONEPEZIL MACROCYCLIC COMPOUND PHOSPHATE BUFFERED SALINE THIOCHOLINE ACETYLCHOLINESTERASE BERBERINE CARBOFURAN CARBON IMMOBILIZED ENZYME METHYLENE BLUE PHOSPHATE ACETYLCHOLINESTERASE ACETYLCHOLINESTERASE SENSOR CARBOFURANS DONEPEZIL ELECTROPOLYMERISATION FLOW-THROUGH ANALYSIS FLOWTHROUGH INHIBITOR DETERMINATION PILLAR[5]ARENE THIOCHOLINE ARTICLE CHEMICAL REACTION CONTROLLED STUDY DILUTION ELECTRIC CURRENT ENZYME IMMOBILIZATION ENZYME INHIBITION NONHUMAN PEANUT POINT OF CARE TESTING SPIKE URINE ELECTRODE GENETIC PROCEDURES SOOT ENZYME IMMOBILIZATION |
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| Описание |
Fast and reliable determination of enzyme inhibitors are of great importance in environmental monitoring and biomedicine because of the high biological activity and toxicity of such species and the necessity of their reliable assessment in many media. In this work, a flow-through biosensor has been developed and produced by 3D printing from poly(lactic acid). Acetylcholinesterase from an electric eel was immobilized on the inner walls of the reactor cell. The concentration of thiocholine formed in enzymatic hydrolysis of the substrate was monitored amperometrically with a screen-printed carbon electrode modified with carbon black particles, pillar[5]arene, electropolymerized Methylene blue and thionine. In the presence of thiocholine, the cathodic current at −0.25 V decreased because of an alternative chemical reaction of the macrocycle. The conditions of enzyme immobilization and signal measurements were optimized and the performance of the biosensor was assessed in the determination of reversible (donepezil, berberine) and irreversible (carbofuran) inhibitors. In the optimal conditions, the flow-through biosensor made it possible to determine 1.0 nM–1.0 μM donepezil, 1.0 μM–1.0 mM berberine and 10 nM to 0.1 μM carbofuran. The AChE biosensor was tested on spiked samples of artificial urine for drugs and peanuts for carbofuran. Possible interference of the sample components was eliminated by dilution of the samples with phosphate buffer. Easy mounting, low cost of replaceable parts of the cell and satisfactory analytical and metrological characteristics made the biosensor a promising future application as a point-of-care or point-of-demand device outside of a chemical laboratory. © 2022 by the authors.
Russian Foundation for Basic Research, РФФИ, (20-33-90107) This research was funded by the Russian Foundation for Basic Research (grant 20-33-90107). |
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| Дата |
2024-04-08T11:07:13Z
2024-04-08T11:07:13Z 2022 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
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| Идентификатор |
Ivanov, A, Stoikov, D, Shafigullina, I, Shurpik, D, Stoikov, I & Evtugyn, G 2022, 'Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor', Biosensors, Том. 12, № 9, 676, стр. 676. https://doi.org/10.3390/bios12090676
Ivanov, A., Stoikov, D., Shafigullina, I., Shurpik, D., Stoikov, I., & Evtugyn, G. (2022). Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor. Biosensors, 12(9), 676. [676]. https://doi.org/10.3390/bios12090676 2079-6374 Final All Open Access; Gold Open Access; Green Open Access https://www.mdpi.com/2079-6374/12/9/676/pdf?version=1663832517 https://www.mdpi.com/2079-6374/12/9/676/pdf?version=1663832517 http://elar.urfu.ru/handle/10995/131424 10.3390/bios12090676 85138417567 000858633100001 |
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| Язык |
en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Biosensors
Biosensors |
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