One-Step Electropolymerization of Azure A and Carbon Nanomaterials for DNA-Sensor Assembling and Doxorubicin Biosensing
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
One-Step Electropolymerization of Azure A and Carbon Nanomaterials for DNA-Sensor Assembling and Doxorubicin Biosensing
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| Автор |
Porfireva, A.
Begisheva, E. Rogov, A. Evtugyn, G. |
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| Тематика |
CARBON BLACK
CARBON NANOTUBES CYCLIC VOLTAMMETRY DOXORUBICIN DETERMINATION ELECTROCHEMICAL DNA SENSOR ELECTROPOLYMERIZATION POLY(AZURE A) |
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| Описание |
New highly sensitive voltammetric DNA-sensors have been developed for the detection of cytostatic drug doxorubicin based on Azure A electropolymerized on various carbon nanomaterials, i.e., functionalized multi-walled carbon nanotubes (fMWCNTs) and carbon black (CB). Carbon materials promote electropolymerization of the Azure A dye applied as a matrix for DNA molecules saturated with methylene blue (MB) molecules. Interaction with the intercalator (doxorubicin) liberates the MB molecules and changes redox activity. The doxorubicin concentration ranges reached by cyclic voltammetry were from 0.1 pM to 100 nM (limit of detection, LOD, 0.03 pM) for the biosensor based on CB, and from 0.3 pM to 0.1 nM (LOD 0.3 pM) for that based on fMWCNTs. DNA-sensors were tested on spiked samples of artificial serum, and biological and pharmaceutical samples. The DNA-sensors can find further application in the monitoring of the doxorubicin residuals in cancer treatment, as well as for pharmacokinetics studies. © 2022 by the authors.
Russian Foundation for Basic Research, РФФИ, (20-03-00207) Russian Science Foundation, RSF, (17-73-20024) Funding: This research was funded by Russian Science Foundation (optimization of the DNA sensor design and doxorubicin measurements), grant number 17-73-20024, and Russian Foundation for Basic Research (electropolymerization from the surface layer of the suspension formed by carbon materials), grant number 20-03-00207. |
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| Дата |
2024-04-08T11:07:59Z
2024-04-08T11:07:59Z 2022 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
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| Идентификатор |
Porfireva, A, Begisheva, E, Rogov, A & Evtugyn, G 2022, 'One-Step Electropolymerization of Azure A and Carbon Nanomaterials for DNA-Sensor Assembling and Doxorubicin Biosensing', C-Journal of Carbon Research, Том. 8, № 4, 75. https://doi.org/10.3390/c8040075
Porfireva, A., Begisheva, E., Rogov, A., & Evtugyn, G. (2022). One-Step Electropolymerization of Azure A and Carbon Nanomaterials for DNA-Sensor Assembling and Doxorubicin Biosensing. C-Journal of Carbon Research, 8(4), [75]. https://doi.org/10.3390/c8040075 2311-5629 Final All Open Access; Gold Open Access https://www.mdpi.com/2311-5629/8/4/75/pdf?version=1670486799 https://www.mdpi.com/2311-5629/8/4/75/pdf?version=1670486799 http://elar.urfu.ru/handle/10995/131549 10.3390/c8040075 85160433708 000900577000001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//17-73-20024
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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Multidisciplinary Digital Publishing Institute (MDPI)
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| Источник |
C
C-Journal of Carbon Research |
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