Peculiarities of the Acetylcholine Action on the Contractile Function of Cardiomyocytes from the Left and Right Atria in Rats
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
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Peculiarities of the Acetylcholine Action on the Contractile Function of Cardiomyocytes from the Left and Right Atria in Rats
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| Автор |
Butova, X.
Myachina, T. Simonova, R. Kochurova, A. Bozhko, Y. Arkhipov, M. Solovyova, O. Kopylova, G. Shchepkin, D. Khokhlova, A. |
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| Тематика |
ACETYLCHOLINE
ACTIN–MYOSIN INTERACTION LEFT AND RIGHT ATRIA PHOSPHORYLATION OF SARCOMERIC PROTEINS SARCOMERE SHORTENING SINGLE CARDIOMYOCYTES [CA2+]I TRANSIENTS ACETYLCHOLINE ACTINS ANIMALS HEART ATRIA MYOCYTES, CARDIAC MYOSIN LIGHT CHAINS RATS SARCOMERES ACETYLCHOLINE CALCIUM CHANNEL CARDIAC MYOSIN F ACTIN MYOSIN HEAVY CHAIN TROPONIN TROPONIN I ACETYLCHOLINE ACTIN MYOSIN LIGHT CHAIN ACTIN MYOSIN INTERACTION ACTION POTENTIAL ANIMAL EXPERIMENT ANIMAL MODEL ARTICLE CALCIUM HOMEOSTASIS CARDIAC MUSCLE CELL CELL VIABILITY CHOLINERGIC SYSTEM CONTROLLED STUDY DEPOLARIZATION HEART ATRIUM HEART FUNCTION HEART MUSCLE CONTRACTILITY KINETICS LIGHT CHAIN MALE NONHUMAN PROTEIN FUNCTION PROTEIN PURIFICATION RAT SARCOMERE LENGTH VAGUS NERVE STIMULATION WHOLE CELL PATCH CLAMP ANIMAL CARDIAC MUSCLE CELL HEART ATRIUM METABOLISM SARCOMERE |
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| Описание |
Acetylcholine (ACh) is the neurotransmitter of the parasympathetic nervous system that modulates cardiac function, and its high concentrations may induce atrial fibrillation. We compared the ACh action on the mechanical function of single cardiomyocytes from the left atria (LA) and the right atria (RA). We exposed single rat LA and RA cardiomyocytes to 1, 10, and 100 µM ACh for 10–15 min and measured the parameters of sarcomere shortening–relengthening and cytosolic calcium ([Ca2+]i) transients during cell contractions. We also studied the effects of ACh on cardiac myosin function using an in vitro motility assay and analyzed the phosphorylation level of sarcomeric proteins. In LA cardiomyocytes, ACh decreased the time to peak sarcomere shortening, time to 50% relengthening, and time to peak [Ca2+]i transients. In RA cardiomyocytes, ACh affected the time of shortening and relengthening only at 10 µM. In the in vitro motility assay, ACh reduced to a greater extent the sliding velocity of F-actin over myosin from LA cardiomyocytes, which was accompanied by a more pronounced decrease in phosphorylation of the myosin regulatory light chain (RLC) in LA cardiomyocytes than in RA cardiomyocytes. Our findings indicate that ACh plays an important role in modulating the contractile function of LA and RA, provoking more pronounced changes in the time course of sarcomere shortening–relengthening and the kinetics of actin–myosin interaction in LA cardiomyocytes. © 2022 by the authors.
Institute of Immunology and Physiology Ural State Medical University, (122022200089-4) The study was supported by State Program No 121040500248-7 (Ural State Medical University) and State Program No 122022200089-4 (Institute of Immunology and Physiology). The work was performed using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology. |
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| Дата |
2024-04-08T11:08:01Z
2024-04-08T11:08:01Z 2022 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
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| Идентификатор |
Butova, X, Myachina, T, Simonova, R, Kochurova, A, Bozhko, Y, Arkhipov, M, Solovyova, O, Kopylova, G, Shchepkin, D & Khokhlova, A 2022, 'Peculiarities of the Acetylcholine Action on the Contractile Function of Cardiomyocytes from the Left and Right Atria in Rats', Cells, Том. 11, № 23, 3809. https://doi.org/10.3390/cells11233809
Butova, X., Myachina, T., Simonova, R., Kochurova, A., Bozhko, Y., Arkhipov, M., Solovyova, O., Kopylova, G., Shchepkin, D., & Khokhlova, A. (2022). Peculiarities of the Acetylcholine Action on the Contractile Function of Cardiomyocytes from the Left and Right Atria in Rats. Cells, 11(23), [3809]. https://doi.org/10.3390/cells11233809 2073-4409 Final All Open Access; Gold Open Access; Green Open Access https://www.mdpi.com/2073-4409/11/23/3809/pdf?version=1669976932 https://www.mdpi.com/2073-4409/11/23/3809/pdf?version=1669976932 http://elar.urfu.ru/handle/10995/131558 10.3390/cells11233809 85143657873 000896537100001 |
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en
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ |
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application/pdf
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MDPI
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| Источник |
Cells
Cells |
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