The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl
Электронный научный архив УРФУ
Информация об архиве | Просмотр оригинала| Поле | Значение | |
| Заглавие |
The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl
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| Автор |
Gerasimova, E.
Gazizullina, E. Kolbaczkaya, S. Ivanova, A. |
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| Тематика |
2,2′-DIPHENYL-1-PICRYLHYDRAZYL
ANTIOXIDANT CAPACITY POTENTIOMETRY |
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| Описание |
For the first time, new possibilities of using the DPPH• as a signal-forming oxidant molecule with potentiometric detection are shown. The CV method confirmed the presence of a quasi-reversible potential-determining system DPPH•/DPPH-H under experimental conditions. This fact makes it possible to use DPPH• as the model of the oxidizing agent for obtaining an analytical signal by the potentiometry method. The potentiometric approach makes it possible to obtain the value of the Nernst slope and the antioxidant capacity in one experiment. It consists of an antioxidant supplement and two consecutive DPPH• supplements. In this case, the calculation of the Nernst slope is carried out by introducing the second addition of the oxidizing agent and constructing a calibration curve against the reaction background with an antioxidant. Solutions of individual antioxidants α-tocopherol, quercetin, (±)-catechin hydrate, and α-lipoic acid were studied by the developed approach. A high correlation with the results of spectrophotometric measurements is shown. At the same time, the potentiometry method is devoid of the concentration limitations of the spectrophotometric method, which was confirmed. In the study of plant materials extracts, a high correlation of antioxidant capacity, obtained by potentiometric and spectrophotometric methods, was shown only for objects whose color did not contribute to the DPPH• absorption. The versatility of the potentiometric method for studying objects of any color was shown. © 2022 by the authors.
Russian Science Foundation, RSF, (20-13-00142) This work was supported by the Russian Science Foundation (Project No. 20-13-00142). |
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| Дата |
2024-04-08T11:08:10Z
2024-04-08T11:08:10Z 2022 |
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| Тип |
Article
Journal article (info:eu-repo/semantics/article) Published version (info:eu-repo/semantics/publishedVersion) |
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| Идентификатор |
Gerasimova, E, Gazizullina, E, Kolbaczkaya, S & Ivanova, A 2022, 'The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl', Antioxidants, Том. 11, № 10, 1974. https://doi.org/10.3390/antiox11101974
Gerasimova, E., Gazizullina, E., Kolbaczkaya, S., & Ivanova, A. (2022). The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl. Antioxidants, 11(10), [1974]. https://doi.org/10.3390/antiox11101974 2076-3921 Final All Open Access; Gold Open Access; Green Open Access https://www.mdpi.com/2076-3921/11/10/1974/pdf?version=1664634691 https://www.mdpi.com/2076-3921/11/10/1974/pdf?version=1664634691 http://elar.urfu.ru/handle/10995/131586 10.3390/antiox11101974 85140451750 000872023500001 |
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| Язык |
en
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| Связанные ресурсы |
info:eu-repo/grantAgreement/RSF//20-13-00142
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| Права |
Open access (info:eu-repo/semantics/openAccess)
cc-by https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ |
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| Формат |
application/pdf
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| Издатель |
MDPI
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| Источник |
Antioxidants
Antioxidants |
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