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New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley)

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Заглавие New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley)
 
Автор Darkazanli, M.
Kiseleva, I.
 
Тематика CORE LOSS
FIELD-ORIENTED CONTROL
INDUCTION MACHINE
OPTIMIZED CONTROL
SUCKER ROD PUMP
VARIABLE SPEED DRIVE
 
Описание The aim of this study was to the isolate the endophytic bacteria, optimize its isolation procedure. Ethanol, sodium hypochlorite, and mercuric chloride at various concentrations and duration were employed to optimize the surface sterilization for the isolation of endophytes from Phaseolus vulgaris, Pisum sativum, and Hordeum vulgare. A total of 21 endophytic bacteria have been isolated from three plants. Combination of 2% sodium hypochlorite, 70% ethanol, and 0.1% mercuric chloride was found effective for the surface sterilization of Phaseolus vulgaris and Pisum sativum. In case of Hordeum vulgare, 70% ethanol and 2% sodium hypochlorite was found suitable for the surface sterilization. Ethanol, sodium hypochlorite, and mercuric chloride were found effective decontaminating agents in optimum condition. © 2021 Author(s).
Department of Science and Technology, Ministry of Science and Technology, India, डीएसटी, (ʋ 19-516-45006)
Russian Foundation for Basic Research, РФФИ
Government Council on Grants, Russian Federation
Funding text 1: The work was funded by RFBR and DST according to the research project ʋ 19-516-45006. The authors also
Funding text 2: acknowledge financial support by the Government of the Russian Federation, Act 211, contract ʋ 02.A03.21.0006.
 
Дата 2024-04-22T15:53:20Z
2024-04-22T15:53:20Z
2021
 
Тип Conference paper
Conference object (info:eu-repo/semantics/conferenceObject)
Published version (info:eu-repo/semantics/publishedVersion)
 
Идентификатор Darkazanli, M & Kiseleva, I 2021, New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley). в GV Zyryanov, S Santra & OS Taniya (ред.), Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020: Proceedings of the IV International Conference., 040010, AIP Conference Proceedings, Том. 2388, American Institute of Physics Inc., 4th International Conference on Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020, Yekaterinburg, Российская Федерация, 16/11/2020. https://doi.org/10.1063/5.0069149
Darkazanli, M., & Kiseleva, I. (2021). New surface sterilization protocols for isolation of endophytic bacteria from plants (black turtle beans, peas, and barley). в G. V. Zyryanov, S. Santra, & O. S. Taniya (Ред.), Modern Synthetic Methodologies for Creating Drugs and Functional Materials, MOSM 2020: Proceedings of the IV International Conference [040010] (AIP Conference Proceedings; Том 2388). American Institute of Physics Inc.. https://doi.org/10.1063/5.0069149
978-073544142-2
0094-243X
Final
All Open Access; Bronze Open Access
https://aip.scitation.org/doi/pdf/10.1063/5.0069149
https://aip.scitation.org/doi/pdf/10.1063/5.0069149
http://elar.urfu.ru/handle/10995/132425
49872425
10.1063/5.0069149
85120405728
001123654800013
 
Язык en
 
Права Open access (info:eu-repo/semantics/openAccess)
cc-by-nc
 
Формат application/pdf
 
Издатель American Institute of Physics Inc.
 
Источник AIP Conference Proceedings
AIP Conference Proceedings